Development of a novel near-infrared fluorescent probe based on nile blue for the detection of leucine aminopeptidase as a cancer biomarker
Por:
M. DOMÍNGUEZ, D. AZORÍN-SORIANO, J. GARCÍA-FLEITAS, J. SOLER-BEATTY, A. BERNARDOS, A. GARCÍA-FERNÁNDEZ, J. BLANDEZ, F. SANCENÓN and R. MARTÍNEZ-MÁÑEZ
Publicada:
1 sep 2025
Ahead of Print:
1 abr 2025
Resumen:
We report herein the design and synthesis of a near-infrared fluorescent probe (NB-SO3-Leu) derived from the Nile Blue (NB) fluorophore scaffold, modified with sulfonic groups to improve solubility and biocompatibility. The mechanism of action of the NB-SO3-Leu probe was successfully validated in the presence of leucine aminopeptidase (LAP), a widely known cancer biomarker. The fluorescence signal of NB-SO3-Leu, which was initially low, increased 4-fold after incubation with the LAP enzyme after 15 min. In addition, the NB-SO3-Leu probe was validated in vitro in 4T1 (mouse mammary carcinoma cells), HeLa (human cervical cancer cells), and SK-Mel-103 (human melanoma cells) with high endogenous LAP levels. This work aims to advance in the development of a new generation of reliable and sensitive cancer detection methods.
Filiaciones:
M. DOMÍNGUEZ:
Univ Valencia, Univ Politecn Valencia, Inst Interuniv Invest Reconocimiento Mol & Desarro, Camino Vera S-N, Valencia 46022, Spain
Inst Salud Carlos III, CIBER Bioingn Biomat & Nanomed CIBER BBN, Madrid 28029, Spain
D. AZORÍN-SORIANO:
Univ Valencia, Univ Politecn Valencia, Inst Interuniv Invest Reconocimiento Mol & Desarro, Camino Vera S-N, Valencia 46022, Spain
Univ Politecn Valencia, Ctr Invest Principe Felipe, Unidad Mixta UPV CIPF Invest Mecanismos Enfermedad, C Eduardo Primo Yufera 3, Valencia 46100, Spain
J. GARCÍA-FLEITAS:
Univ Valencia, Univ Politecn Valencia, Inst Interuniv Invest Reconocimiento Mol & Desarro, Camino Vera S-N, Valencia 46022, Spain
J. SOLER-BEATTY:
Univ Valencia, Univ Politecn Valencia, Inst Interuniv Invest Reconocimiento Mol & Desarro, Camino Vera S-N, Valencia 46022, Spain
Univ Politecn Valencia, Ctr Invest Principe Felipe, Unidad Mixta UPV CIPF Invest Mecanismos Enfermedad, C Eduardo Primo Yufera 3, Valencia 46100, Spain
:
Univ Valencia, Univ Politecn Valencia, Inst Interuniv Invest Reconocimiento Mol & Desarro, Camino Vera S-N, Valencia 46022, Spain
Inst Salud Carlos III, CIBER Bioingn Biomat & Nanomed CIBER BBN, Madrid 28029, Spain
Univ Politecn Valencia, Ctr Invest Principe Felipe, Unidad Mixta UPV CIPF Invest Mecanismos Enfermedad, C Eduardo Primo Yufera 3, Valencia 46100, Spain
:
Univ Valencia, Univ Politecn Valencia, Inst Interuniv Invest Reconocimiento Mol & Desarro, Camino Vera S-N, Valencia 46022, Spain
Inst Salud Carlos III, CIBER Bioingn Biomat & Nanomed CIBER BBN, Madrid 28029, Spain
Univ Politecn Valencia, Ctr Invest Principe Felipe, Unidad Mixta UPV CIPF Invest Mecanismos Enfermedad, C Eduardo Primo Yufera 3, Valencia 46100, Spain
:
Univ Valencia, Univ Politecn Valencia, Inst Interuniv Invest Reconocimiento Mol & Desarro, Camino Vera S-N, Valencia 46022, Spain
Inst Salud Carlos III, CIBER Bioingn Biomat & Nanomed CIBER BBN, Madrid 28029, Spain
Univ Politecn Valencia, Unidad Mixta Invest Nanomed & Sensores, Inst Invest Sanitaria La Fe IISLAFE, Ave Fernando Abril Martorell 106, Valencia 46026, Spain
Univ Cambridge, Dept Chem Engn & Biotechnol, Philippa Fawcett Dr, Cambridge CB3 0AS, England
F. SANCENÓN:
Univ Valencia, Univ Politecn Valencia, Inst Interuniv Invest Reconocimiento Mol & Desarro, Camino Vera S-N, Valencia 46022, Spain
Inst Salud Carlos III, CIBER Bioingn Biomat & Nanomed CIBER BBN, Madrid 28029, Spain
Univ Politecn Valencia, Ctr Invest Principe Felipe, Unidad Mixta UPV CIPF Invest Mecanismos Enfermedad, C Eduardo Primo Yufera 3, Valencia 46100, Spain
Univ Politecn Valencia, Unidad Mixta Invest Nanomed & Sensores, Inst Invest Sanitaria La Fe IISLAFE, Ave Fernando Abril Martorell 106, Valencia 46026, Spain
:
Univ Valencia, Univ Politecn Valencia, Inst Interuniv Invest Reconocimiento Mol & Desarro, Camino Vera S-N, Valencia 46022, Spain
Inst Salud Carlos III, CIBER Bioingn Biomat & Nanomed CIBER BBN, Madrid 28029, Spain
Univ Politecn Valencia, Ctr Invest Principe Felipe, Unidad Mixta UPV CIPF Invest Mecanismos Enfermedad, C Eduardo Primo Yufera 3, Valencia 46100, Spain
Univ Politecn Valencia, Unidad Mixta Invest Nanomed & Sensores, Inst Invest Sanitaria La Fe IISLAFE, Ave Fernando Abril Martorell 106, Valencia 46026, Spain
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