microRNAs-mediated regulation of insulin signaling in white adipose tissue during aging: Role of caloric restriction.
Por:
Corrales P, Martin-Taboada M, Vivas-García Y, Torres L, Ramirez-Jimenez L, Lopez Y, Horrillo D, Vila-Bedmar R, Barber-Cano E, Izquierdo-Lahuerta A, Peña-Chilet M, Martínez C, Dopazo J, Ros M and Medina-Gomez G
Publicada:
4 jul 2023
Ahead of Print:
4 jul 2023
Resumen:
Caloric restriction is a non-pharmacological intervention known to ameliorate the metabolic defects associated with aging, including insulin resistance. The levels of miRNA expression may represent a predictive tool for aging-related alterations. In order to investigate the role of miRNAs underlying insulin resistance in adipose tissue during the early stages of aging, 3- and 12-month-old male animals fed ad libitum, and 12-month-old male animals fed with a 20% caloric restricted diet were used. In this work we demonstrate that specific miRNAs may contribute to the impaired insulin-stimulated glucose metabolism specifically in the subcutaneous white adipose tissue, through the regulation of target genes implicated in the insulin signaling cascade. Moreover, the expression of these miRNAs is modified by caloric restriction in middle-aged animals, in accordance with the improvement of the metabolic state. Overall, our work demonstrates that alterations in posttranscriptional gene expression because of miRNAs dysregulation might represent an endogenous mechanism by which insulin response in the subcutaneous fat depot is already affected at middle age. Importantly, caloric restriction could prevent this modulation, demonstrating that certain miRNAs could constitute potential biomarkers of age-related metabolic alterations.
Filiaciones:
Corrales P:
Department of Basic Sciences of Health, Area of Biochemistry and Molecular Biology, Universidad Rey Juan Carlos, Alcorcon, Madrid, Spain
Martin-Taboada M:
Department of Basic Sciences of Health, Area of Biochemistry and Molecular Biology, Universidad Rey Juan Carlos, Alcorcon, Madrid, Spain
Vivas-García Y:
Department of Basic Sciences of Health, Area of Biochemistry and Molecular Biology, Universidad Rey Juan Carlos, Alcorcon, Madrid, Spain
Metabolism and Cell Signalling Laboratory, Spanish National Cancer Research Centre, Madrid, Spain
Torres L:
Department of Basic Sciences of Health, Area of Biochemistry and Molecular Biology, Universidad Rey Juan Carlos, Alcorcon, Madrid, Spain
Ramirez-Jimenez L:
Genomics and Genetics Unit, Centro de Investigación Príncipe Felipe, Valencia, Spain
Lopez Y:
Department of Basic Sciences of Health, Area of Biochemistry and Molecular Biology, Universidad Rey Juan Carlos, Alcorcon, Madrid, Spain
Horrillo D:
Department of Basic Sciences of Health, Area of Biochemistry and Molecular Biology, Universidad Rey Juan Carlos, Alcorcon, Madrid, Spain
Vila-Bedmar R:
Department of Basic Sciences of Health, Area of Biochemistry and Molecular Biology, Universidad Rey Juan Carlos, Alcorcon, Madrid, Spain
Barber-Cano E:
Genomics and Genetics Unit, Centro de Investigación Príncipe Felipe, Valencia, Spain
Izquierdo-Lahuerta A:
Department of Basic Sciences of Health, Area of Biochemistry and Molecular Biology, Universidad Rey Juan Carlos, Alcorcon, Madrid, Spain
Peña-Chilet M:
Platform for Computational Medicine, Fundación Progreso y Salud, Sevilla, Spain
Systems and Computational Medicine Unit, Biomedical Institute of Seville (IBiS), Sevilla, Spain
Plataforma BiER, Consorcio de Investigaciones Biomédicas en Red en Enfermedades Raras (CIBERER), Sevilla, Spain
Martínez C:
Department of Basic Sciences of Health, Area of Biochemistry and Molecular Biology, Universidad Rey Juan Carlos, Alcorcon, Madrid, Spain
:
Platform for Computational Medicine, Fundación Progreso y Salud, Sevilla, Spain
Systems and Computational Medicine Unit, Biomedical Institute of Seville (IBiS), Sevilla, Spain
Plataforma BiER, Consorcio de Investigaciones Biomédicas en Red en Enfermedades Raras (CIBERER), Sevilla, Spain
Ros M:
Department of Basic Sciences of Health, Area of Biochemistry and Molecular Biology, Universidad Rey Juan Carlos, Alcorcon, Madrid, Spain
Medina-Gomez G:
Department of Basic Sciences of Health, Area of Biochemistry and Molecular Biology, Universidad Rey Juan Carlos, Alcorcon, Madrid, Spain
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