Lipofuscin labeling through biorthogonal strain-promoted azide-alkyne cycloaddition for the detection of senescent cells
Por:
B. LOZANO-TORRES, J. BLANDEZ, A. GARCIA-FERNANDEZ, F. SANCENON and R. MARTINEZ-MANEZ
Publicada:
1 mar 2023
Ahead of Print:
1 may 2022
Resumen:
A new method for senescent cell detection is described, which is based on lipofuscin labeling with a fluorescent reporter through a biorthogonal strain-promoted azide-alkyne cycloaddition. The sensing protocol involves a first step where the interaction of lipofuscin with a Sudan Black B derivative containing an azide moiety (SBB-N-3) is carried out. In the final step, the azide moiety reacts with a fluorophore containing a cyclooctene ring (BODIPY). The efficacy of this two-step protocol is assessed in senescent melanoma SK-MEL-103 cells, senescent triple-negative breast cancer MDA-MB-231 cells and senescent WI-38 fibroblasts. In all cases, a clear fluorescence pattern was observed in senescent cells, compared to proliferative cells, only when the SBB-N-3-BODIPY probe was formed. Our results provide an alternative tool for the detection of senescent cells, based on an in situ bio-orthogonal reaction for lipofuscin labeling.
Filiaciones:
:
Univ Valencia, Univ Politecn Valencia, Inst Interuniv Invest Reconocimiento Mol & Desarr, Camino Vera S-N, Valencia 46022, Spain
Univ Politecn Valencia, Ctr Invest Principe Felipe, Unidad Mixta UPV CIPF Invest Mecanismos Enfermeda, Valencia, Spain
CIBER Bioingn Biomat & Nanomed CIBER BBN, Madrid, Spain
:
Univ Valencia, Univ Politecn Valencia, Inst Interuniv Invest Reconocimiento Mol & Desarr, Camino Vera S-N, Valencia 46022, Spain
CIBER Bioingn Biomat & Nanomed CIBER BBN, Madrid, Spain
Univ Politecn Valencia, IIS La Fe, Unidad Mixta Invest Nanomed & Sensores, Valencia, Spain
:
Univ Valencia, Univ Politecn Valencia, Inst Interuniv Invest Reconocimiento Mol & Desarr, Camino Vera S-N, Valencia 46022, Spain
Univ Politecn Valencia, Ctr Invest Principe Felipe, Unidad Mixta UPV CIPF Invest Mecanismos Enfermeda, Valencia, Spain
CIBER Bioingn Biomat & Nanomed CIBER BBN, Madrid, Spain
:
Univ Valencia, Univ Politecn Valencia, Inst Interuniv Invest Reconocimiento Mol & Desarr, Camino Vera S-N, Valencia 46022, Spain
Univ Politecn Valencia, Ctr Invest Principe Felipe, Unidad Mixta UPV CIPF Invest Mecanismos Enfermeda, Valencia, Spain
CIBER Bioingn Biomat & Nanomed CIBER BBN, Madrid, Spain
Univ Politecn Valencia, IIS La Fe, Unidad Mixta Invest Nanomed & Sensores, Valencia, Spain
:
Univ Valencia, Univ Politecn Valencia, Inst Interuniv Invest Reconocimiento Mol & Desarr, Camino Vera S-N, Valencia 46022, Spain
Univ Politecn Valencia, Ctr Invest Principe Felipe, Unidad Mixta UPV CIPF Invest Mecanismos Enfermeda, Valencia, Spain
CIBER Bioingn Biomat & Nanomed CIBER BBN, Madrid, Spain
Univ Politecn Valencia, IIS La Fe, Unidad Mixta Invest Nanomed & Sensores, Valencia, Spain
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