Lactose-Gated Mesoporous Silica Particles for Intestinal Controlled Delivery of Essential Oil Components: An In Vitro and In Vivo Study
Por:
E. POYATOS-RACIONERO, I. GONZALEZ-ALVAREZ, P. SANCHEZ-MORENO, L. SITIA, F. GATTO, P. POMPA, E. AZNAR, M. GONZALEZ-ALVAREZ, R. MARTINEZ-MANEZ, M. MARCOS and A. BERNARDOS
Publicada:
1 jul 2021
Resumen:
Mesoporous silica microparticles functionalized with lactose for the specific release of essential oil components (EOCs) in the small intestine are presented. In vitro and in vivo intestinal models were applied to validate the microparticles (M41-EOC-L), in which the presence of lactase acts as the triggering stimulus for the controlled release of EOCs. Among the different microdevices prepared (containing thymol, eugenol and cinnamaldehyde), the one loaded with cinnamaldehyde showed the most significant Caco-2 cell viability reduction. On the other hand, interaction of the particles with enterocyte-like monolayers showed a reduction of EOCs permeability when protected into the designed microdevices. Then, a microdevice loaded with cinnamaldehyde was applied in the in vivo model of Wistar rat. The results showed a reduction in cinnamaldehyde plasma levels and an increase in its concentration in the lumen of the gastrointestinal tract (GIT). The absence of payload release in the stomach, the progressive release throughout the intestine and the prolonged stay of the payload in the GIT-lumen increased the bioavailability of the encapsulated compound at the site of the desired action. These innovative results, based on the specific intestinal controlled delivery, suggest that the M41-payload-L could be a potential hybrid microdevice for the protection and administration of bioactive molecules in the small intestine and colon.
Filiaciones:
E. POYATOS-RACIONERO:
Univ Valencia, Univ Politecn Valencia, Inst Interuniv Invest Reconocimiento Mol & Desarr, Camino Vera S-N, Valencia 46022, Spain
CIBER Bioingn Biomat & Nanomed CIBER BBN, Valencia 46022, Spain
I. GONZALEZ-ALVAREZ:
Univ Miguel Hernandez, Dept Ingn, Secc Farm & Tecnol Farmaceut, Alicante 03550, Spain
P. SANCHEZ-MORENO:
Univ Granada, Fac Sci, Dept Appl Phys, Ave Fuente Nueva S-N, Granada 18071, Spain
L. SITIA:
Univ Milan, Dept Biomed & Clin Sci Luigi Sacco, Nanomed Lab, I-20157 Milan, Italy
F. GATTO:
Ist Italiano Tecnol IIT, Nanobiointeract & Nanodiagnost, Via Morego 30, I-16163 Genoa, Italy
P. POMPA:
Ist Italiano Tecnol IIT, Nanobiointeract & Nanodiagnost, Via Morego 30, I-16163 Genoa, Italy
:
Univ Valencia, Univ Politecn Valencia, Inst Interuniv Invest Reconocimiento Mol & Desarr, Camino Vera S-N, Valencia 46022, Spain
CIBER Bioingn Biomat & Nanomed CIBER BBN, Valencia 46022, Spain
Univ Politecn Valencia, Inst Invest Sanitaria La Fe, Unidad Mixta Invest Nanomed & Sensores, Av Fernando Abril Martorell 106, Valencia 46026, Spain
Univ Politecn Valencia, Ctr Invest Principe Felipe, Unidad Mixta UPV CIPF Invest Mecanismos Enfermeda, C Eduardo Primo Yufera 3, Valencia 46100, Spain
M. GONZALEZ-ALVAREZ:
Univ Miguel Hernandez, Dept Ingn, Secc Farm & Tecnol Farmaceut, Alicante 03550, Spain
:
Univ Valencia, Univ Politecn Valencia, Inst Interuniv Invest Reconocimiento Mol & Desarr, Camino Vera S-N, Valencia 46022, Spain
CIBER Bioingn Biomat & Nanomed CIBER BBN, Valencia 46022, Spain
Univ Politecn Valencia, Inst Invest Sanitaria La Fe, Unidad Mixta Invest Nanomed & Sensores, Av Fernando Abril Martorell 106, Valencia 46026, Spain
Univ Politecn Valencia, Ctr Invest Principe Felipe, Unidad Mixta UPV CIPF Invest Mecanismos Enfermeda, C Eduardo Primo Yufera 3, Valencia 46100, Spain
Univ Politecn Valencia, Dept Quim, Camino Vera S-N, Valencia 46022, Spain
:
Univ Valencia, Univ Politecn Valencia, Inst Interuniv Invest Reconocimiento Mol & Desarr, Camino Vera S-N, Valencia 46022, Spain
CIBER Bioingn Biomat & Nanomed CIBER BBN, Valencia 46022, Spain
Univ Politecn Valencia, Inst Invest Sanitaria La Fe, Unidad Mixta Invest Nanomed & Sensores, Av Fernando Abril Martorell 106, Valencia 46026, Spain
Univ Politecn Valencia, Ctr Invest Principe Felipe, Unidad Mixta UPV CIPF Invest Mecanismos Enfermeda, C Eduardo Primo Yufera 3, Valencia 46100, Spain
Univ Politecn Valencia, Dept Quim, Camino Vera S-N, Valencia 46022, Spain
:
Univ Valencia, Univ Politecn Valencia, Inst Interuniv Invest Reconocimiento Mol & Desarr, Camino Vera S-N, Valencia 46022, Spain
CIBER Bioingn Biomat & Nanomed CIBER BBN, Valencia 46022, Spain
Univ Politecn Valencia, Ctr Invest Principe Felipe, Unidad Mixta UPV CIPF Invest Mecanismos Enfermeda, C Eduardo Primo Yufera 3, Valencia 46100, Spain
Univ Politecn Valencia, Dept Quim, Camino Vera S-N, Valencia 46022, Spain
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