The Effectiveness of Glutathione Redox Status as a Possible Tumor Marker in Colorectal Cancer
Por:
D. ACEVEDO-LEON, L. MONZO-BELTRAN, S. GOMEZ-ABRIL, N. ESTAN-CAPELL, N. CAMARASA-LILLO, M. PEREZ-EBRI, J. ESCANDON-ALVAREZ, E. ALONSO-IGLESIAS, M. SANTAOLARIA-AYORA, A. CARBONELL-MONCHO, J. VENTURA-GAYETE, L. PLA, M. MARTINEZ-BISBAL, R. MARTINEZ-MANEZ, L. BAGAN-DEBON, A. VINA-ALMUNIA, M. MARTINEZ-SANTAMARIA, M. RUIZ-LUQUE, J. ALONSO-FERNANDEZ, C. BANULS and G. SAEZ
Publicada:
1 jun 2021
Resumen:
The role of oxidative stress (OS) in cancer is a matter of great interest due to the implication of reactive oxygen species (ROS) and their oxidation products in the initiation of tumorigenesis, its progression, and metastatic dissemination. Great efforts have been made to identify the mechanisms of ROS-induced carcinogenesis; however, the validation of OS byproducts as potential tumor markers (TMs) remains to be established. This interventional study included a total of 80 colorectal cancer (CRC) patients and 60 controls. By measuring reduced glutathione (GSH), its oxidized form (GSSG), and the glutathione redox state in terms of the GSSG/GSH ratio in the serum of CRC patients, we identified significant changes as compared to healthy subjects. These findings are compatible with the effectiveness of glutathione as a TM. The thiol redox state showed a significant increase towards oxidation in the CRC group and correlated significantly with both the tumor state and the clinical evolution. The sensitivity and specificity of serum glutathione levels are far above those of the classical TMs CEA and CA19.9. We conclude that the GSSG/GSH ratio is a simple assay which could be validated as a novel clinical TM for the diagnosis and monitoring of CRC.
Filiaciones:
D. ACEVEDO-LEON:
Hosp Univ Dr Peset FISABIO, Serv Anal Clin, Valencia 46017, Spain
L. MONZO-BELTRAN:
Univ Valencia, Fac Med & Odontotol INCLIVA, Dept Bioquim & Biol Mol, Valencia 46010, Spain
S. GOMEZ-ABRIL:
Hosp Univ Dr Peset FISABIO, Serv Cirugia Gen & Aparato Digest, Valencia 46017, Spain
N. ESTAN-CAPELL:
Hosp Univ Dr Peset FISABIO, Serv Anal Clin, Valencia 46017, Spain
N. CAMARASA-LILLO:
Hosp Univ Dr Peset FISABIO, Serv Anat Patol, Valencia 46017, Spain
M. PEREZ-EBRI:
Hosp Univ Dr Peset FISABIO, Serv Anat Patol, Valencia 46017, Spain
J. ESCANDON-ALVAREZ:
Hosp Univ Dr Peset FISABIO, Serv Anat Patol, Valencia 46017, Spain
E. ALONSO-IGLESIAS:
Univ Valencia, Fac Med & Odontotol INCLIVA, Dept Bioquim & Biol Mol, Valencia 46010, Spain
M. SANTAOLARIA-AYORA:
Hosp Univ Dr Peset FISABIO, Serv Anal Clin, Valencia 46017, Spain
A. CARBONELL-MONCHO:
Hosp Univ Dr Peset FISABIO, Serv Anal Clin, Valencia 46017, Spain
J. VENTURA-GAYETE:
Hosp Univ Dr Peset FISABIO, Serv Anal Clin, Valencia 46017, Spain
L. PLA:
Univ Valencia, Univ Politecn Valencia, Inst Interuniv Invest Reconocimiento Mol & Desarr, Valencia 46022, Spain
CIBER Bioingn Biomat & Nanomed CIBER BBN, Madrid 28029, Spain
:
Univ Valencia, Univ Politecn Valencia, Inst Interuniv Invest Reconocimiento Mol & Desarr, Valencia 46022, Spain
CIBER Bioingn Biomat & Nanomed CIBER BBN, Madrid 28029, Spain
Univ Politecn Valencia, Ctr Invest Principe Felipe, Unidad Mixta UPV CIPF Invest Mecanismos Enfermeda, Valencia 46012, Spain
Univ Politecn Valencia, Inst Invest Sanitaria La Fe, Unidad Mixta Invest Nanomed & Sensores, Valencia 46026, Spain
Univ Politecn Valencia, Dept Quim, Valencia 46022, Spain
Univ Valencia, Dept Quim Fis, Valencia 46100, Spain
:
Univ Valencia, Univ Politecn Valencia, Inst Interuniv Invest Reconocimiento Mol & Desarr, Valencia 46022, Spain
CIBER Bioingn Biomat & Nanomed CIBER BBN, Madrid 28029, Spain
Univ Politecn Valencia, Ctr Invest Principe Felipe, Unidad Mixta UPV CIPF Invest Mecanismos Enfermeda, Valencia 46012, Spain
Univ Politecn Valencia, Inst Invest Sanitaria La Fe, Unidad Mixta Invest Nanomed & Sensores, Valencia 46026, Spain
Univ Politecn Valencia, Dept Quim, Valencia 46022, Spain
Univ Valencia, Dept Quim Fis, Valencia 46100, Spain
L. BAGAN-DEBON:
Fac Med & Odontol INCLIVA, Dept Estomatol, Valencia 46010, Spain
A. VINA-ALMUNIA:
Univ Valencia, Consorcio Hosp Gen, Ctr Salud San Isidro, Valencia 46014, Spain
M. MARTINEZ-SANTAMARIA:
Hosp Univ Dr Peset FISABIO, Serv Anal Clin, Valencia 46017, Spain
M. RUIZ-LUQUE:
Hosp Univ Dr Peset FISABIO, Serv Anal Clin, Valencia 46017, Spain
J. ALONSO-FERNANDEZ:
Hosp Univ Dr Peset FISABIO, Serv Anal Clin, Valencia 46017, Spain
C. BANULS:
Hosp Univ Dr Peset FISABIO, Serv Endocrinol & Nutr, Valencia 46017, Spain
G. SAEZ:
Hosp Univ Dr Peset FISABIO, Serv Anal Clin, Valencia 46017, Spain
Univ Valencia, Fac Med & Odontotol INCLIVA, Dept Bioquim & Biol Mol, Valencia 46010, Spain
gold, Green Published
|