Mapping the expression of transient receptor potential channels across murine placental development
Por:
K. DE CLERCQ, V. PEREZ-GARCIA, R. VAN BREE, F. POLLASTRO, K. PEERAER, T. VOETS and J. VRIENS
Publicada:
1 jun 2021
Ahead of Print:
1 abr 2021
Resumen:
Transient receptor potential (TRP) channels play prominent roles in ion homeostasis by their ability to control cation influx. Mouse placentation is governed by the processes of trophoblast proliferation, invasion, differentiation, and fusion, all of which require calcium signaling. Although certain TRP channels have been shown to contribute to maternal-fetal transport of magnesium and calcium, a role for TRP channels in specific trophoblast functions has been disregarded. Using qRT-PCR and in situ hybridisation, the spatio-temporal expression pattern of TRP channels in the mouse placenta across gestation (E10.5-E18.5) was assessed. Prominent expression was observed for Trpv2, Trpm6, and Trpm7. Calcium microfluorimetry in primary trophoblast cells isolated at E14.5 of gestation further revealed the functional activity of TRPV2 and TRPM7. Finally, comparing TRP channels expression in mouse trophoblast stem cells (mTSCs) and mouse embryonic stem cells (mESC) confirmed the specific expression of TRPV2 during placental development. Moreover, TRP channel expression was similar in mTSCs compared to primary trophoblasts and validate mTSC as a model to study TRP channels in placental development. Collectivity, our results identify a specific spatio-temporal TRP channel expression pattern in trophoblasts, suggesting a possible involvement in regulating the process of placentation.
Filiaciones:
K. DE CLERCQ:
Katholieke Univ Leuven, Lab Endometrium Endometriosis & Reprod Med, Dept Dev & Regenerat, Herestr 49,Box 611, B-3000 Leuven, Belgium
Katholieke Univ Leuven, Lab Ion Channel Res, Dept Cellular & Mol Med, VIB Ctr Brain & Dis Res, Herestr 49,Box 802, B-3000 Leuven, Belgium
:
Univ Cambridge, Dept Physiol Dev & Neurosci, Ctr Trophoblast Res, Downing St, Cambridge CB2 3EG, England
Babraham Inst, Epigenet Programme, Babraham Res Campus, Cambridge CB22 3AT, England
Ctr Invest Principe Felipe, Mol Basis Human Dis, Eduardo Primo Yufera 3, Valencia 46012, Spain
R. VAN BREE:
Katholieke Univ Leuven, Lab Endometrium Endometriosis & Reprod Med, Dept Dev & Regenerat, Herestr 49,Box 611, B-3000 Leuven, Belgium
F. POLLASTRO:
Univ Piemonte Orientale Amedeo, Dipartimento Sci Farmaco, I-13100 Vercelli, Italy
K. PEERAER:
Katholieke Univ Leuven, Lab Endometrium Endometriosis & Reprod Med, Dept Dev & Regenerat, Herestr 49,Box 611, B-3000 Leuven, Belgium
T. VOETS:
Katholieke Univ Leuven, Lab Ion Channel Res, Dept Cellular & Mol Med, VIB Ctr Brain & Dis Res, Herestr 49,Box 802, B-3000 Leuven, Belgium
J. VRIENS:
Katholieke Univ Leuven, Lab Endometrium Endometriosis & Reprod Med, Dept Dev & Regenerat, Herestr 49,Box 611, B-3000 Leuven, Belgium
Hybrid Gold
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