Chromo-fluorogenic probes for beta-galactosidase detection
Por:
B. LOZANO-TORRES, J. BLANDEZ, F. SANCENON and R. MARTINEZ-MANEZ
Publicada:
1 abr 2021
Ahead of Print:
1 feb 2021
Resumen:
beta-Galactosidase (beta-Gal) is a widely used enzyme as a reporter gene in the field of molecular biology which hydrolyzes the beta-galactosides into monosaccharides. beta-Gal is an essential enzyme in humans and its deficiency or its overexpression results in several rare diseases. Cellular senescence is probably one of the most relevant physiological disorders that involve beta-Gal enzyme. In this review, we assess the progress made to date in the design of molecular-based probes for the detection of beta-Gal both in vitro and in vivo. Most of the reported molecular probes for the detection of beta-Gal consist of a galactopyranoside residue attached to a signalling unit through glycosidic bonds. The beta-Gal-induced hydrolysis of the glycosidic bonds released the signalling unit with remarkable changes in color and/or emission. Additional examples based on other approaches are also described. The wide applicability of these probes for the rapid and in situ detection of de-regulation beta-Gal-related diseases has boosted the research in this fertile field.
Filiaciones:
:
Univ Valencia, Univ Politecn Valencia, Inst Interuniv Invest Reconocimiento Mol & Desarr, Camino Vera S-N, Valencia 46022, Spain
Univ Politecn Valencia, Unidad Mixta UPV CIPF Invest Mecanismos Enfermeda, Ctr Invest Principe Felipe, Valencia 46022, Spain
CIBER Bioingn Biomat & Nanomed CIBER BBN, Barcelona, Spain
Univ Politecn Valencia, IIS La Fe, Unidad Mixta Invest Nanomed & Sensores, Valencia 46026, Spain
J. BLANDEZ:
Univ Valencia, Univ Politecn Valencia, Inst Interuniv Invest Reconocimiento Mol & Desarr, Camino Vera S-N, Valencia 46022, Spain
Univ Politecn Valencia, Unidad Mixta UPV CIPF Invest Mecanismos Enfermeda, Ctr Invest Principe Felipe, Valencia 46022, Spain
CIBER Bioingn Biomat & Nanomed CIBER BBN, Barcelona, Spain
Univ Politecn Valencia, IIS La Fe, Unidad Mixta Invest Nanomed & Sensores, Valencia 46026, Spain
:
Univ Valencia, Univ Politecn Valencia, Inst Interuniv Invest Reconocimiento Mol & Desarr, Camino Vera S-N, Valencia 46022, Spain
Univ Politecn Valencia, Unidad Mixta UPV CIPF Invest Mecanismos Enfermeda, Ctr Invest Principe Felipe, Valencia 46022, Spain
CIBER Bioingn Biomat & Nanomed CIBER BBN, Barcelona, Spain
Univ Politecn Valencia, IIS La Fe, Unidad Mixta Invest Nanomed & Sensores, Valencia 46026, Spain
:
Univ Valencia, Univ Politecn Valencia, Inst Interuniv Invest Reconocimiento Mol & Desarr, Camino Vera S-N, Valencia 46022, Spain
Univ Politecn Valencia, Unidad Mixta UPV CIPF Invest Mecanismos Enfermeda, Ctr Invest Principe Felipe, Valencia 46022, Spain
CIBER Bioingn Biomat & Nanomed CIBER BBN, Barcelona, Spain
Univ Politecn Valencia, IIS La Fe, Unidad Mixta Invest Nanomed & Sensores, Valencia 46026, Spain
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