Human adipose-derived mesenchymal stem cells accelerate decellularized neobladder regeneration
Por:
V. MORENO-MANZANO, M. MELLADO-LOPEZ, M. MORERA-ESTEVE, A. ALASTRUE-AGUDO, V. BISBAL-VELASCO, J. FORTEZA-VILA, A. SERRANO-AROCA and C. VERA-DONOSO
Publicada:
1 mar 2020
Resumen:
Decellularized natural bladder matrices (neobladders) represent an exciting means to regenerate the bladder following bladder cancer-associated cystectomy. In this study, we compare the evolution of decellularized matrices with recellularized matrices by seeding it with human adipose-derived mesenchymal stem cells (ADSC) after implantation following partial cystectomy in rats. We discovered significant anatomical differences since 10 days after neobladder implantation with the ADSC-containing matrices promoting a significant recovery of mature p63- and cytokeratin 7-positive urothelium. We also discovered significantly induced expression of the vimentin mesoderm marker in the submucosal layer in ADSC-seeded matrices. Interestingly, we found a higher expression of smooth muscle actin in transversal and longitudinal smooth muscle layers with ADSC-seeded matrices. Furthermore, ADSC also showed increased vascularization and nerve innervation of the neobladder as determined by the distribution of CD31 and S100 beta reactivity, respectively. We believe that ADSC and their paracrine-acting pro-regenerative secretome within decellularized matrices represent an efficient bladder substitution strategy; however, we require a fuller understanding of the mechanisms involved before clinical studies can begin.
Filiaciones:
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Ctr Invest Principe Felipe, Neuronal & Tissue Regenerat Lab, Valencia, Spain
Univ Catal Valencia San Vicente Martir, Fac Vet & Ciencias Experimentales, C Guillem Castro 94, Valencia 46001, Spain
:
Ctr Invest Principe Felipe, Neuronal & Tissue Regenerat Lab, Valencia, Spain
M. MORERA-ESTEVE:
Univ CEU, Vet Fac, Surg Dept, Valencia, Spain
:
Ctr Invest Principe Felipe, Neuronal & Tissue Regenerat Lab, Valencia, Spain
:
Ctr Invest Principe Felipe, Neuronal & Tissue Regenerat Lab, Valencia, Spain
:
Univ Catolica Valencia, Mol Pathol & Translat Res Oncol, Unidad Mixta, Valencia, Spain
Ctr Invest Principe Felipe, Valencia, Spain
A. SERRANO-AROCA:
Univ Catal Valencia San Vicente Martir, Fac Vet & Ciencias Experimentales, C Guillem Castro 94, Valencia 46001, Spain
C. VERA-DONOSO:
La Fe Univ, Dept Urol, Valencia 46026, Spain
La Fe Univ & Polytech Hosp, Dept Urol, Valencia 46026, Spain
Hosp La Fe, Hlth Res Inst, Valencia 46026, Spain
Gold, Green Published
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