Evidences for Expression and Location of ANGPTL8 in Human Adipose Tissue.
Por:
Catalano-Iniesta L, Sánchez Robledo V, Iglesias-Osma MC, Galán Albiñana A, Carrero S, Blanco EJ, Carretero-Hernández M, Carretero J and García-Barrado MJ
Publicada:
13 feb 2020
Resumen:
The metabolism of triglycerides (TGs) is regulated, among others, by the lipoprotein lipase (LPL) that hydrolyses the TGs on endothelial cells. In turn, LPL is inhibited by the ANGPTLs family of proteins, such as ANGPTL3, 4, and, 8; the latter is the least known. In this work, we have tried to establish the expression and localisation of the Angiopoietin-like 8 (ANGPTL8) protein in the visceral adipose tissue (VAT) of morbid-obese and non-obese patients. 109 subjects (66 women and 43 men) undergoing laparoscopic surgery participated in this study. A blood sample and a portion of the VAT were obtained, and the patients were classified according to their Body Mass Index (BMI) as non-obese (19.5-30 kg/m 2 ) and morbid-obese (40-50 kg/m 2 ). No significant changes in ANGPTL8 plasma levels were determined by EIA in obese patients. The immunocytochemistry and Western blotting showed the presence of increased ANGPTL8 in morbid-obese patients ( p < 0.05). In-situ hybridisation and a real time polymerase chain reaction (RT-PCR) confirmed that the mRNA that encodes ANGPTL8 was present in adipocytes, without differences in their nutritional state ( p = 0.89), and even in the endothelial cells. Our data suggests that ANGPT8 plasmatic levels do not change significantly in patients with morbid obesity, although there is a modest difference related to gender. Besides, we demonstrate that in visceral adipose tissue, ANGPTL8 is well defined in the cytoplasm of adipocytes coexisting with perilipin-1 and its mRNA, also is present in endothelial cells. These findings suggest the possibility that among other functions, ANGPTL8 could perform either a paracrine and/or an endocrine role in the adipose tissue.
Filiaciones:
Catalano-Iniesta L:
Department of Physiology and Pharmacology, Faculty of Medicine, University of Salamanca, 38007 Salamanca, Spain
Laboratory of Neuroendocrinology, Institute of Neurosciences of Castilla y León (INCyL), and Laboratory of Neuroendocrinology and Obesity, Institute of Biomedical Research of Salamanca (IBSAL), University of Salamanca, 38007 Salamanca, Spain
Sánchez Robledo V:
Department of Physiology and Pharmacology, Faculty of Medicine, University of Salamanca, 38007 Salamanca, Spain
Laboratory of Neuroendocrinology, Institute of Neurosciences of Castilla y León (INCyL), and Laboratory of Neuroendocrinology and Obesity, Institute of Biomedical Research of Salamanca (IBSAL), University of Salamanca, 38007 Salamanca, Spain
Iglesias-Osma MC:
Department of Physiology and Pharmacology, Faculty of Medicine, University of Salamanca, 38007 Salamanca, Spain
Laboratory of Neuroendocrinology, Institute of Neurosciences of Castilla y León (INCyL), and Laboratory of Neuroendocrinology and Obesity, Institute of Biomedical Research of Salamanca (IBSAL), University of Salamanca, 38007 Salamanca, Spain
:
Gene Expression and RNA Metabolism Laboratory, Centro de Investigación Príncipe Felipe (CIPF), C/ E. Primo Yúfera 3, 46012 Valencia, Spain
Carrero S:
Surgery Service of University Hospital of Salamanca, 38007 Salamanca, Spain
Blanco EJ:
Laboratory of Neuroendocrinology, Institute of Neurosciences of Castilla y León (INCyL), and Laboratory of Neuroendocrinology and Obesity, Institute of Biomedical Research of Salamanca (IBSAL), University of Salamanca, 38007 Salamanca, Spain
Department of Human Anatomy and Histology, Faculty of Medicine, University of Salamanca, 38007 Salamanca, Spain
Carretero-Hernández M:
Department of Human Anatomy and Histology, Faculty of Medicine, University of Salamanca, 38007 Salamanca, Spain
Carretero J:
Laboratory of Neuroendocrinology, Institute of Neurosciences of Castilla y León (INCyL), and Laboratory of Neuroendocrinology and Obesity, Institute of Biomedical Research of Salamanca (IBSAL), University of Salamanca, 38007 Salamanca, Spain
Department of Human Anatomy and Histology, Faculty of Medicine, University of Salamanca, 38007 Salamanca, Spain
García-Barrado MJ:
Department of Physiology and Pharmacology, Faculty of Medicine, University of Salamanca, 38007 Salamanca, Spain
Laboratory of Neuroendocrinology, Institute of Neurosciences of Castilla y León (INCyL), and Laboratory of Neuroendocrinology and Obesity, Institute of Biomedical Research of Salamanca (IBSAL), University of Salamanca, 38007 Salamanca, Spain
Gold, Green Published
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