A Long Noncoding RNA Regulates Sister Chromatid Cohesion
Por:
F. MARCHESE, E. GROSSI, O. MARIN-BEJAR, S. BHARTI, I. RAIMONDI, J. GONZALEZ, D. MARTINEZ-HERRERA, A. ATHIE, A. AMADOZ, R. BROSH and M. HUARTE
Publicada:
4 ago 2016
Resumen:
Long noncoding RNAs (lncRNAs) are involved in diverse cellular processes through multiple mechanisms. Here, we describe a previously uncharacterized human lncRNA, CONCR (cohesion regulator noncoding RNA), that is transcriptionally activated by MYC and is upregulated in multiple cancer types. The expression of CONCR is cell cycle regulated, and it is required for cell-cycle progression and DNA replication. Moreover, cells depleted of CONCR show severe defects in sister chromatid cohesion, suggesting an essential role for CONCR in cohesion establishment during cell division. CONCR interacts with and regulates the activity of DDX11, a DNA-dependent ATPase and helicase involved in DNA replication and sister chromatid cohesion. These findings unveil a direct role for an lncRNA in the establishment of sister chromatid cohesion by modulating DDX11 enzymatic activity.
Filiaciones:
F. MARCHESE:
Univ Navarra, Dept Gene Therapy & Regulat Gene Express, Ctr Appl Med Res CIMA, 55 Pio 12 Ave, Pamplona 31008, Spain
Inst Hlth Res Navarra IdiSNA, Pamplona 31008, Spain
E. GROSSI:
Univ Navarra, Dept Gene Therapy & Regulat Gene Express, Ctr Appl Med Res CIMA, 55 Pio 12 Ave, Pamplona 31008, Spain
Inst Hlth Res Navarra IdiSNA, Pamplona 31008, Spain
O. MARIN-BEJAR:
Univ Navarra, Dept Gene Therapy & Regulat Gene Express, Ctr Appl Med Res CIMA, 55 Pio 12 Ave, Pamplona 31008, Spain
Inst Hlth Res Navarra IdiSNA, Pamplona 31008, Spain
S. BHARTI:
NIA, Lab Mol Gerontol, NIH, Biomed Res Ctr, 251 Bayview Blvd, Baltimore, MD 21224 USA
I. RAIMONDI:
Univ Navarra, Dept Gene Therapy & Regulat Gene Express, Ctr Appl Med Res CIMA, 55 Pio 12 Ave, Pamplona 31008, Spain
Inst Hlth Res Navarra IdiSNA, Pamplona 31008, Spain
J. GONZALEZ:
Univ Navarra, Dept Gene Therapy & Regulat Gene Express, Ctr Appl Med Res CIMA, 55 Pio 12 Ave, Pamplona 31008, Spain
Inst Hlth Res Navarra IdiSNA, Pamplona 31008, Spain
D. MARTINEZ-HERRERA:
Univ Navarra, Dept Gene Therapy & Regulat Gene Express, Ctr Appl Med Res CIMA, 55 Pio 12 Ave, Pamplona 31008, Spain
Inst Hlth Res Navarra IdiSNA, Pamplona 31008, Spain
A. ATHIE:
Univ Navarra, Dept Gene Therapy & Regulat Gene Express, Ctr Appl Med Res CIMA, 55 Pio 12 Ave, Pamplona 31008, Spain
Inst Hlth Res Navarra IdiSNA, Pamplona 31008, Spain
:
Univ Navarra, Dept Gene Therapy & Regulat Gene Express, Ctr Appl Med Res CIMA, 55 Pio 12 Ave, Pamplona 31008, Spain
Inst Hlth Res Navarra IdiSNA, Pamplona 31008, Spain
CIPF, Computat Genom Dept, Valencia 46012, Spain
R. BROSH:
NIA, Lab Mol Gerontol, NIH, Biomed Res Ctr, 251 Bayview Blvd, Baltimore, MD 21224 USA
M. HUARTE:
Univ Navarra, Dept Gene Therapy & Regulat Gene Express, Ctr Appl Med Res CIMA, 55 Pio 12 Ave, Pamplona 31008, Spain
Inst Hlth Res Navarra IdiSNA, Pamplona 31008, Spain
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