Mapping of six somatic linker histone H1 variants in human breast cancer cells uncovers specific features of H1.2
Por:
L. MILLAN-ARINO, A. ISLAM, A. IZQUIERDO-BOULDSTRIDGE, R. MAYOR, J. TERME, N. LUQUE, M. SANCHO, N. LOPEZ-BIGAS and A. JORDAN
Publicada:
1 abr 2014
Resumen:
Seven linker histone H1 variants are present in human somatic cells with distinct prevalence across cell types. Despite being key structural components of chromatin, it is not known whether the different variants have specific roles in the regulation of nuclear processes or are differentially distributed throughout the genome. Using variant-specific antibodies to H1 and hemagglutinin (HA)-tagged recombinant H1 variants expressed in breast cancer cells, we have investigated the distribution of six H1 variants in promoters and genome-wide. H1 is depleted at promoters depending on its transcriptional status and differs between variants. Notably, H1.2 is less abundant than other variants at the transcription start sites of inactive genes, and promoters enriched in H1.2 are different from those enriched in other variants and tend to be repressed. Additionally, H1.2 is enriched at chromosomal domains characterized by low guanine-cytosine (GC) content and is associated with lamina-associated domains. Meanwhile, other variants are associated with higher GC content, CpG islands and gene-rich domains. For instance, H1.0 and H1X are enriched at gene-rich chromosomes, whereas H1.2 is depleted. In short, histone H1 is not uniformly distributed along the genome and there are differences between variants, H1.2 being the one showing the most specific pattern and strongest correlation with low gene expression.
Filiaciones:
L. MILLAN-ARINO:
Inst Biol Mol Barcelona IBMB CSIC, Dept Mol Genom, E-08028 Barcelona, Spain
A. ISLAM:
Univ Pompeu Fabra, Res Programme Biomed Informat, E-08003 Barcelona, Spain
Univ Dhaka, Dept Genet Engn, Dhaka 1000, Bangladesh
A. IZQUIERDO-BOULDSTRIDGE:
Inst Biol Mol Barcelona IBMB CSIC, Dept Mol Genom, E-08028 Barcelona, Spain
R. MAYOR:
Inst Biol Mol Barcelona IBMB CSIC, Dept Mol Genom, E-08028 Barcelona, Spain
J. TERME:
Inst Biol Mol Barcelona IBMB CSIC, Dept Mol Genom, E-08028 Barcelona, Spain
N. LUQUE:
Inst Biol Mol Barcelona IBMB CSIC, Dept Mol Genom, E-08028 Barcelona, Spain
:
Ctr Invest Principe Felipe, E-46012 Valencia, Spain
N. LOPEZ-BIGAS:
Univ Pompeu Fabra, Res Programme Biomed Informat, E-08003 Barcelona, Spain
Inst Catalana Recerca & Estudis Avancats ICREA, E-08010 Barcelona, Spain
A. JORDAN:
Inst Biol Mol Barcelona IBMB CSIC, Dept Mol Genom, E-08028 Barcelona, Spain
Green Published, gold, Green Submitted
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