Assessing PTEN Subcellular Localization
Por:
A. GIL, J. LOPEZ and R. PULIDO
Publicada:
1 ene 2016
Resumen:
PTEN subcellular localization is fundamental in the execution of the distinct PTEN biological activities, including not only its PI(3,4,5)P-3 phosphatase activity when associated to membranes but also its subcellular compartment-specific interactions with regulatory and effector proteins, including those exerted in the nucleus. As a consequence, PTEN subcellular localization is tightly regulated in vivo by both intrinsic and extrinsic mechanisms. The plasma membrane/nucleus/cytoplasm partitioning of PTEN has been the focus of several studies, both from a mechanistic and from a disease-association point of view. Here, we summarize the current knowledge on PTEN plasma membrane/nucleus/cytoplasm distribution, and present subcellular fractionation, immunofluorescence, and immunohistochemical methods to study the distribution and shuttling of PTEN between these subcellular compartments.
Filiaciones:
:
Ctr Invest Principe Felipe, Valencia, Spain
Biomed Res Inst INCLIVA, Dept Hematol & Med Oncol, Valencia, Spain
J. LOPEZ:
Univ Basque Country UPV EHU, Cruces Univ Hosp, Dept Pathol, Baracaldo, Spain
Biocruces Hlth Res Inst, Baracaldo, Bizkaia, Spain
:
Ctr Invest Principe Felipe, Valencia, Spain
Biocruces Hlth Res Inst, Baracaldo, Bizkaia, Spain
Basque Fdn Sci, Ikerbasque, Bilbao, Spain
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