Apaf1 inhibition promotes cell recovery from apoptosis
Por:
A. GORTAT, M. SANCHO, L. MONDRAGON, A. MESSEGUER, E. PEREZ-PAYA and M. ORZAEZ
Publicada:
1 nov 2015
Resumen:
The protein apoptotic protease activating factor 1 (Apaf1) is the central component of the apoptosome, a multiprotein complex that activates procaspase-9 after cytochrome c release from the mitochondria in the intrinsic pathway of apoptosis. We have developed a vital method that allows fluorescence-activated cell sorting of cells at different stages of the apoptotic pathway and demonstrated that upon pharmacological inhibition of Apaf1, cells recover from doxorubicin- or hypoxia-induced early apoptosis to normal healthy cell. Inhibiting Apaf1 not only prevents procaspase-9 activation but delays massive mitochondrial damage allowing cell recovery.
Filiaciones:
A. GORTAT:
Ctr Invest Principe Felipe, Lab Peptide & Prot Chem, Valencia 46012, Spain
:
Ctr Invest Principe Felipe, Lab Peptide & Prot Chem, Valencia 46012, Spain
L. MONDRAGON:
Ctr Invest Principe Felipe, Lab Peptide & Prot Chem, Valencia 46012, Spain
A. MESSEGUER:
Inst Quim Avanzada Cataluna CSIC, Dept Chem & Biomol Nanotechnol, Barcelona 08034, Spain
:
Ctr Invest Principe Felipe, Lab Peptide & Prot Chem, Valencia 46012, Spain
IBV CSIC, Inst Biomed Valencia, Valencia 46010, Spain
:
Ctr Invest Principe Felipe, Lab Peptide & Prot Chem, Valencia 46012, Spain
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