Bax transmembrane domain interacts with prosurvival Bcl-2 proteins in biological membranes
Por:
V. ANDREU-FERNANDEZ, M. SANCHO, A. GENOVES, E. LUCENDO, F. TODT, J. LAUTERWASSER, K. FUNK, G. JAHREIS, E. PEREZ-PAYA, I. MINGARRO, F. EDLICH and M. ORZAEZ
Publicada:
10 ene 2017
Resumen:
The Bcl-2 (B-cell lymphoma 2) protein Bax (Bcl-2 associated X, apoptosis regulator) can commit cells to apoptosis via outer mitochondrial membrane permeabilization. Bax activity is controlled in healthy cells by prosurvival Bcl-2 proteins. C-terminal Bax transmembrane domain interactions were implicated recently in Bax pore formation. Here, we show that the isolated transmembrane domains of Bax, Bcl-xL (B-cell lymphoma-extra large), and Bcl-2 can mediate interactions between Bax and prosurvival proteins inside the membrane in the absence of apoptotic stimuli. Bcl-2 protein transmembrane domains specifically homooligomerize and heterooligomerize in bacterial and mitochondrial membranes. Their interactions participate in the regulation of Bcl-2 proteins, thus modulating apoptotic activity. Our results suggest that interactions between the transmembrane domains of Bax and antiapoptotic Bcl-2 proteins represent a previously unappreciated level of apoptosis regulation.
Filiaciones:
V. ANDREU-FERNANDEZ:
Cent Invest Principe Felipe, Lab Peptide & Prot Chem, E-46012 Valencia, Spain
Univ Freiburg, Inst Biochem & Mol Biol, D-79104 Freiburg, Germany
Univ Valencia, Estruct Recerca Interdisciplinar Biotecnol & Biom, Dept Bioquim & Biol Mol, E-46100 Burjassot, Spain
:
Cent Invest Principe Felipe, Lab Peptide & Prot Chem, E-46012 Valencia, Spain
A. GENOVES:
Cent Invest Principe Felipe, Lab Peptide & Prot Chem, E-46012 Valencia, Spain
:
Cent Invest Principe Felipe, Lab Peptide & Prot Chem, E-46012 Valencia, Spain
F. TODT:
Univ Freiburg, Inst Biochem & Mol Biol, D-79104 Freiburg, Germany
J. LAUTERWASSER:
Univ Freiburg, Inst Biochem & Mol Biol, D-79104 Freiburg, Germany
Univ Freiburg, Fac Biol, D-79104 Freiburg, Germany
K. FUNK:
Univ Freiburg, Inst Biochem & Mol Biol, D-79104 Freiburg, Germany
Univ Freiburg, Fac Biol, D-79104 Freiburg, Germany
G. JAHREIS:
Cent Invest Principe Felipe, Lab Peptide & Prot Chem, E-46012 Valencia, Spain
Martin Luther Univ Halle Wittenberg, Dept Biochem Biotechnol, D-06120 Halle, Germany
:
Cent Invest Principe Felipe, Lab Peptide & Prot Chem, E-46012 Valencia, Spain
CSIC, Inst Biomed Valencia, E-46010 Valencia, Spain
I. MINGARRO:
Univ Valencia, Estruct Recerca Interdisciplinar Biotecnol & Biom, Dept Bioquim & Biol Mol, E-46100 Burjassot, Spain
F. EDLICH:
Univ Freiburg, Inst Biochem & Mol Biol, D-79104 Freiburg, Germany
Univ Freiburg, Ctr Biol Signaling Studies, BIOSS, D-79104 Freiburg, Germany
:
Cent Invest Principe Felipe, Lab Peptide & Prot Chem, E-46012 Valencia, Spain
Green Published, Bronze
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