Design of Poly-l-Glutamate-Based Complexes for pDNA Delivery
Por:
A. NINO-PARIENTE, A. ARMINAN, S. REINHARD, C. SCHOLZ, E. WAGNER and M. VICENT
Publicada:
1 oct 2017
Resumen:
Due to the polyanionic nature of DNA, typically cationic or neutral delivery vehicles have been used for gene delivery. As a new approach, this study focuses on the design, development, and validation of nonviral polypeptide-based carriers for oligonucleotide delivery based on a negatively charged poly-l-glutamic acid (PGA) backbone partly derivatized with oligoaminoamide residues. To this end, PGA-derivatives modified with different pentameric succinyl tetraethylene pentamines (Stp(5)) are designed. Optionally, histidines for modulation of endosomal buffer capacity and cysteines for pDNA complex stabilization are included, followed by characterization of biophysical properties and gene transfer efficiency in N2a neuroblastoma or 4T1 breast cancer cells.
Filiaciones:
A. NINO-PARIENTE:
CIPF, Polymer Therapeut Lab, C Eduardo Primo Yufera 3, Valencia 46012, Spain
:
CIPF, Polymer Therapeut Lab, C Eduardo Primo Yufera 3, Valencia 46012, Spain
S. REINHARD:
Ludwig Maximilians Univ Munchen, Ctr Nanosci, Dept Pharm, Pharmaceut Biotechnol, Butenandtstr 5-13,Bldg D, D-81377 Munich, Germany
C. SCHOLZ:
Ludwig Maximilians Univ Munchen, Ctr Nanosci, Dept Pharm, Pharmaceut Biotechnol, Butenandtstr 5-13,Bldg D, D-81377 Munich, Germany
E. WAGNER:
Ludwig Maximilians Univ Munchen, Ctr Nanosci, Dept Pharm, Pharmaceut Biotechnol, Butenandtstr 5-13,Bldg D, D-81377 Munich, Germany
:
CIPF, Polymer Therapeut Lab, C Eduardo Primo Yufera 3, Valencia 46012, Spain
Bronze
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